By Anonymous on Tuesday, February 8, 2000 - 03:28 pm:
Does anyone use electrochemical detection to measure glucosamine? I would like to know what kind of reproducibility aand stability of sample solution can one get. I'm using Dionex's method of 30mM NaOH mobile phase and carboPac column.
By Chris Pohl on Wednesday, February 23, 2000 - 05:01 pm:
Glucosamine is widely analyzed and detected using high pH anion exchange with electrochemical detection. Typically the eluent used is approximately 16 mM, so your eluent seems a bit concentrated. Reproducibility should be in the 1-2% range. Sterile sample solution stability is in the multi-month range but with microbial contamination solution stability can be an issue. The general practice is to prepare a standard solution, dispense aliquots into autosampler vials or other suitable containers and freeze the standard. Standard solution vials are simply thawed out immediately before use.
By Anonymous on Monday, February 28, 2000 - 03:55 pm:
Chris Pohl,
Thanks for your response. I would like to discuss with you about the above issue. My telephone no. is 714-934-3191 (PST) or I can call you if you give me your tel. no.
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