By Derek M. Greer on Friday, February 11, 2000 - 07:52 am:
I am using a Brownlee polypore CA column to seperate sugars. the rentention time of lactose, sucrose and maltose are almost identical. I am using water with 1% ACN as a moblile phase. I have tried slowing the flow rate and even using 0.05M Ca acetate to improve selectivity.anyone got a good method for this separation, or know of where to find one?
Thanks!
Derek Greer
By Anonymous on Friday, February 11, 2000 - 09:07 am:
Open up a catalog from any of the column manufacturers, go to the pages on carbohydrate analysis. Many column choices are available for the seperation you discribe. As an example, the "Carbohydrate Analysis Column" from Waters. RT's of Lactose, sucrose and maltose 18.0, 11.9, 15.7 minutes. All of the vendors have these types of columns.
By Anonymous on Friday, February 11, 2000 - 10:07 am:
Try the Supelcosil LC-NH2 (250 x 4.6); 75% acetonitrile at ambient temperature. Maltose, sucrose and lactose are tough on any ion-exclusion column. A warning, some applications show carbohyrates separated on ion exclusion columns at elevated temperatures--as high as 80C. Generally these temperatures are essential for the selectivity.
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