By sarah neary on Wednesday, June 2, 1999 - 07:19 am:
I am trying to establish a HPLC method for determining Ascorbic acid and its derviatives (i.e. ascorbic acid-2-phosphate) in tissue culture medium. Does anyone have any experience in this area?
Many thanks
Sarah.
By B.Buglio on Wednesday, July 7, 1999 - 07:44 pm:
For trace amounts of ascorbic acid in a "soup" you should look into electrochemical(amperometric) detection.