By Ortelli D on Tuesday, February 22, 2000 - 12:51 am:
Dear All,
I'm doing drug analysis in biological fluids (serum, plasma) and I have some problem to destroy quantitavely the binding of drug to protein. Does anyone know how to break this interaction easily. The use of acetonitrile work well but it's not compatible with our analytical system.
Thanks in advance.
By Anonymous on Wednesday, February 23, 2000 - 10:28 am:
Do you inject the plasma/serum directly? Or is there some extraction procedure you use first??
By Ortelli D. on Thursday, February 24, 2000 - 11:05 pm:
the serum is directly injected in the LC-MS system and the mobile phase is not strong enough to break the interaction.
By Uwe Neue on Saturday, March 4, 2000 - 12:52 pm:
The standard approach is to acidify the sample. This should be compatible with your LC/MS method.
How do you know that you have drug binding problems? Maybe the issue is rather the suppression of the ionization of the analyte due to the proteins in your sample....
By Fred on Tuesday, March 7, 2000 - 08:42 am:
Are you using a classical HPLC column ?
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