By Anonymous on Tuesday, March 7, 2000 - 05:43 am:
Hi!
In my lab we're having a discussion about using Nitric acid for cleaning the entire HPLC-system (without column).
Can I use Nitric acid (e.g. 1%) or would this be kiling my system? I use a HP1100 system with normal stainless steel tubing.
By Anonymous on Tuesday, March 7, 2000 - 05:04 pm:
Nitric acid will not harm your system, but unless you have a real solid reason to passivate with nitric, why bother?
By Ana Guillén on Thursday, March 9, 2000 - 09:12 am:
You can use a nítric ácid until 5 % for 15-30 min to clean up your system without problems, but, after that you need to wash it for 48 hours (flow rate 5 ml/min if we are present and 0.2-0.3 ml/min if we aren't)
By Anonymous on Thursday, March 30, 2000 - 09:23 am:
On an HP 1100 system - just make sure you bypass the degasser unit if you have one also. We use a 4N solution flushed for 10 minutes at 1 ml/min. We monitor the UV at 350 nm until the baseline drops. Then we rinse the system with water at 5 ml/min for 1 hour. We check the effluent's pH until it is neutral.
By Anonymous on Friday, March 31, 2000 - 04:01 am:
we are useing 20% NITRIC acid to passivate hplc
system with 3ml flow for 5 minutes. wash system
with water until PH is neutral.
By Anonymous on Tuesday, May 18, 2004 - 02:55 pm:
can i use hydrochloric acid to clean the pumps of HPLC?
By Anonymous on Tuesday, May 18, 2004 - 04:27 pm:
NO!!!!
See:
http://www.goodwin.co.uk/gsc/app_corrosion.htm
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