Hi,
My name is Joke and I'm working on the purification of MAbs (thesissubject). Therefor I use the 'Streamline XL 25'(Pharmacia). To clean the column properly there are some 'clean in place' procedures. In one of the cleaning buffers, we use sarcosyl 5% to eliminate the lypopolysaccharides, which is needed because the purified protein will be used for therapeutic goals. Anytime I clean the column with sarcosyl-containg solution, the fluid above the column becomes all troubled and its filled with flocculents. Most of it can be removed by flushing water trough it but some restants remain in the column. Although this method is recommended by the supplier I doubt teh effectiveness of the use of sarcosyl.