By Anonymous on Sunday, April 16, 2000 - 09:53 am:
What are the different buffer used in LC.
I would like to have some informations for the preparation: the amount (mM),different buffer for different PH acid, basic or neutral.
By Anonymous on Monday, April 17, 2000 - 06:57 pm:
For standard reversed-phase HPLC, the buffer concetrations are around 50 mM. Buffers are used at 1 to 1.5 units around their pKa.
By tom jupille on Monday, April 17, 2000 - 09:01 pm:
Keystone Scientific recently published a very nice guide to buffer preparation. Contact:
Aimee Heyrman
Applications Chemist
Keystone Scientific, Inc.
814-353-2300
aheyrman@keystonescientific.com
You might also want to check out the buffer preparation "wizard" on Zirchrom's site:
http://www.zirchrom.com/buffer/buffer.htm
-- Tom Jupille / LC Resources Inc.
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