By Steph. on Friday, April 28, 2000 - 04:47 am:
Hello,
I'm running a gradient method which uses a starting condition of 50% THF (50% water) at 1ml/min. As I run the initial conditions through the column (Luna C18 5um 4.6 x 50mm) I get such a high backpressure that the system blows the tubing. Does anyone know of any reason for this.
Much appreciated.
By Bruce Freeman on Friday, April 28, 2000 - 12:19 pm:
Are you using any plastic (e.g., PEEK) tubing or hardware? THF attacks many plastics and could easily soften them enough for a tube to slip out of a fitting. The cure is to use all stainless steel hardware.
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