By Monika Felten on Wednesday, June 9, 1999 - 08:34 am:
We purchesed a used Beckman System Gold 166 UV detector. Probable contaminants in flow cell = buffer salts. Anybody know an easy way to clean the flow cell? I am looking for an alternative cleaning method to 20% nitric acid (Beckman recommendation).
By Anonymous on Wednesday, June 9, 1999 - 04:32 pm:
What are the problem symptoms (high pressure? bad light throughput? etc.). If the problem is really just buffer salts, then pulling some warm water through would probably be the best bet (followed by the nitric acid cleaning).
By the way, always *pull* the wash solution thought the flow cell, don't "push".
By Steve on Thursday, June 10, 1999 - 02:39 am:
I'm curious as to why (and how) you would "pull" your wash solution through. Could someone please explain this to me?
Thanks.
By M Bakes on Thursday, June 10, 1999 - 06:29 am:
Steve
Place the inlet of the flow cell in solvent, connect the outlet with appropriate fittings to a syringe, then pull solvent through. The reason cleaning is usually done this way, as I understand, is to prevent fracturing of the flow cell by overpressuring with solvent. I have also used a procedure of connecting a syringe filled with an appropriate wash solution (isopropanol, nitric acid, hot water ...) to the outlet and "back flushing" by pushing the solvent through. Again, be careful not to over-pressure. Hope this answers your questions.
By Anonymous on Tuesday, June 11, 2002 - 10:38 pm:
M Bakes:
Can this warm water be pumped through the flowcell instead of "pulling" it?
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