By Charles Gilman on Tuesday, May 30, 2000 - 02:17 pm:
Can anyone suggest a method for purifying large biomolecules from a cellular matrix? I was told that SEC might not work because of activity loss. Any guidance at all would be appreciated.
By H W Mueller on Wednesday, May 31, 2000 - 12:22 am:
This does not appear to be a problem which you solve just via a suggestion. To aquaintances and me it appears as if each protein, etc., requires an individual study to effect a separation (analysis). Check the primary literature! Also, helpful has been:
J-C Janson, L Ryden, Protein Purification, VCH, 1989 and
The Busy Researcherīs Guide to Biomolecule Chromatography, PerSeptive Biosystems (now PE Biosystems?), also
Methods of Enzymology and, of course, the internet.
By Uwe Neue on Wednesday, May 31, 2000 - 02:04 pm:
Among all the chromatographic separation techniques, SEC is the mildest since there is no interaction with the stationary phase. Therefore I can not see why SEC should cause activity loss. It is also not the most powerful separations technique, since resolution is limited.
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