By Anonymous on Thursday, June 8, 2000 - 03:36 pm:
is there any way other than back flush the column to decrease the pressure of a HPLC column?
By H W Mueller on Thursday, June 8, 2000 - 11:25 pm:
If backflushing doesn´t work, replace the entrance frit (careful!). If proteins, etc., are involved wash until the pressure drops to an acceptable level with 3% Li-dodecylsulfate (aqu.) containing 0.01-0.02M 1,4-dithiothreitol. Incidentally, there are as many methods as chromatographers.
By guido on Friday, September 1, 2000 - 01:20 am:
USE A PRECOLUMN!! I change my precolumn on a regular schedule(every 300 to 500 injections) and i seldom have any trouble with my analytical column. I inject thousands of plasma extracts and peak shape, separation and backpressure are as good as with a new column.
By Uwe Neue on Saturday, September 2, 2000 - 09:09 am:
Applause !!! This is exactly what we are doing and we got column lifetimes exceeding 10,000 injections.....
By Anonymous on Friday, October 27, 2000 - 07:26 am:
If the column is not very expensive, use a new column. The results will be better.
By PGPG on Saturday, October 28, 2000 - 06:38 pm:
Flush with hot water
pgpg@tutopia.com
By Raza on Wednesday, June 2, 2004 - 12:03 am:
Dear Sir,
I would be thankful if you send me information of separation of Rhamnolipids on HPLC.
Thanks,
Raza
NIBGE.
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