Definition:
Resolution
The resolution
between two chromatographic peaks is a measure of how well they are
separated. Resolution is defined as the center-to-center separation
between two peaks divided by the average baseline width of those peaks:
Rs = (tR2 - tR1)/[0.5(wb2 + wb1)]
where tR1 and tR2 are the retention times
of the first and second peaks of interest, and wb1 and wb2 are the baseline widths
of those peaks.
For ideally-shaped
(Gaussian) peaks, a resolution of 1.0 corresponds to approximately 5%
overlap between equal-size peaks. A resolution of 1.5 corresponds to 1%
overlap.