Index

Index

Asymmetry factor
- <2. Tailing or fronting peaks>
- <Definition: asymmetry factor>
- <Definition: tailing factor>
- <Diagnostic: Diluent solvent strength>
- <Diagnostic: Heating and temperature control issues>
- <Diagnostic: Sample overload>
- <Fixing: head space or void>
Back pressure
- <9. Excessive pressure>
- <Fixing: plugged frits>
Bubbles
- <1. Selectivity and/or retention time changes>
- <10. Noisy or drifting baseline>
Column temperature
- <1. Selectivity and/or retention time changes>
- <10. Noisy or drifting baseline>
- <11. Repeatability problems>
- <6. Flat-top peaks>
- <Diagnostic: Heating and temperature control issues>
Contamination
- <8. Extra peaks>
- <9. Excessive pressure>
- <Diagnostic: Gradient garbage>
- <Diagnostic: Late eluters>
Dead time
- <1. Selectivity and/or retention time changes>
- <Definition: capacity factor>
- <Definition: dead time>
Degradation
- <3. Abnormally wide peaks (abnormally low plate count)>
- <7. Missing peaks>
Detector range
- <6. Flat-top peaks>
Drift
- <10. Noisy or drifting baseline>
- <Definition: baseline noise>
Dwell volume
- <1. Selectivity and/or retention time changes>
- <Diagnostic: Measuring dwell volume>
Extra-column volume
- <2. Tailing or fronting peaks>
- <3. Abnormally wide peaks (abnormally low plate count)>
- <4. Peaks with shoulders>
Flow rate
- <1. Selectivity and/or retention time changes>
Gradients
- <8. Extra peaks>
- <Definition: dwell volume>
- <Diagnostic: Gradient garbage>
- <Diagnostic: Gradient step-test>
- <Diagnostic: Measuring dwell volume>
Interference
- <3. Abnormally wide peaks (abnormally low plate count)>
- <4. Peaks with shoulders>
Leaks
- <1. Selectivity and/or retention time changes>
Noise
- <10. Noisy or drifting baseline>
Overload
- <2. Tailing or fronting peaks>
- <6. Flat-top peaks>
Peak splitting
- <5. Split peaks>
Plate count
- <3. Abnormally wide peaks (abnormally low plate count)>
Plate number
- <3. Abnormally wide peaks (abnormally low plate count)>
- <4. Peaks with shoulders>
Pressure
- <9. Excessive pressure>
Pressure fluctuations
- <1. Selectivity and/or retention time changes>
Problems
- <The HPLC Troubleshooting Wizard>
Sample reaction
- <6. Flat-top peaks>
Selectivity
- <1. Selectivity and/or retention time changes>
Selectivity problems
- isocratic, gradient
  - <1. Selectivity and/or retention time changes>
Shoulder
- <4. Peaks with shoulders>
Split peaks
- <5. Split peaks>
System suitability
- <2. Tailing or fronting peaks>
Tailing
- <2. Tailing or fronting peaks>
Tailing factor
- <2. Tailing or fronting peaks>
- Plate Count
  - <4. Peaks with shoulders>
Temperature
- <1. Selectivity and/or retention time changes>
Theoretical plates
- <3. Abnormally wide peaks (abnormally low plate count)>
Time constant
- <10. Noisy or drifting baseline>