<Diagnostic:
Column equilibration time>
Ideally, each
chromatogram will begin with a completely equilibrated column. In
practice, many separations can be run successfully on an incompletely
equilibrated column so long as the degree of equilibration is constant
from run to run.
If you find that the
results from the first run of the day differ from the results of later
runs, try varying the equilibration time between runs (gradient) or
after a change of mobile phase (isocratic).
Ref: LC-GC 17(7)
812 (1999)
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