6.
Flat-top peaks
"Flat-topped" peaks are not
characterized by a single "out-of-specification" measurement. This is a
subjective evaluation on the part of the chromatographer.
The most likely
causes are:
1. Sample
overload.
Generally, this will also be accompanied by severe tailing.
2. Detector
over-range.
3. Sample reaction
on-column.
4. Insufficient
pre-heating of mobile phase when operating at
elevated temperature
5. A
dilution solvent that is stronger than the mobile phase.
© 2006
LC Resources, Inc. all rights reserved