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2018 Schedule

Getting Started in LC
is presented Live on the Web in three 2.5-hour sessions from 8:00 - 10:30 am (Pacific Daylight Time).

September 24, 26, & 28

Register On-line Now!


Live on the Web: Getting Started in Liquid Chromatography

Who should take this course?

Are you new to LC, or do you supervise new users of LC? This on-line course taught by world-class experts is an opportunity to give new users a firm grounding in the fundamentals of LC economically and with minimum disruption to work schedules. "Getting Started in LC" is intended for beginning chromatographers. No hands-on LC experience is required, but attendees should have a solid grasp of basic chemistry.


What does it cover?

"Getting Started in LC" covers the essentials that every scientist needs in order to make effective use of liquid chromatography instrumentation:
  • The basics of the chromatographic process
  • Terms and definitions of chromatography
  • How HPLC systems work
  • Practical tips on preventive maintenance
  • Principles of separation chemistry
  • Why "reversed phase" is the standard separation technique
  • The meaning of "gradients" and why they are used
  • How to get the best quantitation
  • and much more.

What do I need in order to take the course?

  • A personal computer (Windows, Mac, iOS, or Android)
  • A high-speed (broadband) internet connection
  • A phone connection with headset or speakerphone

Here's how it works:

  1. After you register we'll e-mail your course log-in information and password.
  2. Download and print a hard copy of the course handout (approximately 200 pages).
  3. About five minutes before the start of the course, log in to www.gotomeeting.com to download the gotomeeting client and check in for the course. Dial the phone number* for the conference call connection that you were given in your confirmation e-mail.
  4. The course runs a total of six hours in three 2.5-hour sessions .

*Standard long-distance phone rates will apply (approximately $10.00).


What if I can't make it?

All sessions are recorded and will be available for review for a month after the end of the course; if you miss a session, you can catch up at your convenience. If you must cancel, please call at least 48 hours prior to the start of the course in order to receive a refund. No refund will be made for cancellation within 48 hours of the course.


About the instructors
The course was developed by Lloyd Snyder, John Dolan, and Tom Jupille. These sessions will be presented by Tom Jupille.

Registration cost: $947 for a single person. Additional registrants from the same lab are 30% off the regular price; payment via credit card (MC, VISA, or AMEX only).


What topics are covered?

The course is presented in three 2.5-hour sessions "live on the web". This course is interactive, so the exact timing will vary somewhat depending on participants' interests and questions. A typical schedule looks like this:

Section 1. Overview
  • What is HPLC
  • A brief history of HPLC
  • From Apparatus to Instrument

Section 2. Instrumentation:

  • Overview
  • Pumps
  • Injectors & Autosamplers
  • Connecting Tubing & Fittings
  • Column Hardware
  • Detectors

Section 3. HPLC Measurements & Parameters

  • Retention & Selectivity
  • Efficiency
  • Resolution
  • Tailing & Symmetry

Section 4. It's all about chemistry:

  • Reversed-phase
  • Normal-phase & HILIC
  • Ion exchange & Ion pair
  • SEC
  • Gradients

Section 5. Quantitation

  • Integration
  • Calibration

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