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On-Site / Virtual On-Site: Bioanalytical LC-MS/MS

Who should take this course?

This course is designed to provide basic knowledge and practical applications of LC-MS to the analysis of drugs in metabolites in biological extracts. Although no previous experience with HPLC is necessary, the course will be especially valuable to those with some chromatography experience.

What does it cover?

"Bioanalytical LC-MS/MS” is a two-day course explaining the principles of mass spectrometric detection, the design and operation of mass spectrometers, and the interfacing of the MS to the liquid chromatograph to obtain both qualitative and quantitative data. This two-day course covers:

  • MS operation, including the operation of the most popular LC-MS interfaces
  • How a quadrupole mass filter works
  • MS calibration and optimization
  • Ion production, fragmentation and detection
  • Operation in MS, MS-MS and MS-MS-MS modes
  • Structure determination by product ion analysis
  • Quantitative analysis
  • Method development and validation
  • Techniques to get the most from your LC-MS

What will I get from this course?

You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the molecular ion, you will have a solid grounding on which to further build your LC-MS expertise.You will learn how to chose the appropriate separation technique for your application and how to select the best chromatographic mode to meet your separation goals. You will learn how to develop and optimize an LC method and how to troubleshoot it when problems occur. You will learn how to handle columns to achieve good performance and satisfactory lifetime. The focus of this course will be on practical guides and tips to achieve successful biomolecule separations.

When is the course available?

 "The course is presented for groups of 10 - 30 people either "on-site" at your facility or "virtual on-site" using Citrix GoToMeeting service. On-site presentation is two full days. Virtual On-Site is six 2.5-hour sessions (typically 2 or 3 sessions per week). The usual lead time to schedule a course is approximately 4-6 weeks. 

This course is taught as an in-house course only and can be customized to meet your specific needs. To discuss in-house course options, call (971) 259-8195 or send us an e-mail with a brief description of your training needs, and we'll call you with details.

What topics are covered?

The course content can be tailored to your specific needs. A typical schedule looks like this:

Day 1
Quadrupole Physics
Basics of Quadrupole Instrumentation
The Molecular Ion
Calibration and Tubing


Day 2
Qualitative Analysis
Quantitative Analysis
Maintenance and Repair
Regulatory Considerations
Case Studies

MS inlet
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