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Obtaining Separations, Part III: Adjusting Column Conditions
Wait until after you have optimized the mobile phase before you change the column length, particle diameter, or flow rate.
Keywords: method ruggedness, pressure limits, resolution, plate number, particle size, flow rate, column size, extracolumn effects, tradeoffs, selectivity, retention
LC-GC 12(7) 520-524 (1994)
John W. Dolan