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Gradient Elution, Part I: Intuition

In contrast to isocratic separations, gradient elution sometimes can seem to be counter-intuitive. Yet with the proper perspective, the things we intuitively understand about isocratic methods apply in a similar manner to gradients.

keywords: isocratic vs. gradient, gradient time, gradient steepness, gradient range, gradient elution

LCGC 31(3) 204-209 (2013)

John W. Dolan