Gradient Elution, Part III: Surprises

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Gradient Elution, Part III: Surprises

Sometimes when changes are made to gradient conditions, the result isn't what was expected.

keywords: isocratic vs. gradient, column length, column diameter, particle size, flow rate, k*, gradient elution

LCGC 31(5) 382-389 (2013)

John W. Dolan

ERRATUM: The last word on p. 388 should be "decrease" not "increase." A change in particle size from 3 microns to 5 should reduce the pressure, not increase it.