Getting Started in HPLC

Section 0. The Language of HPLC: E

A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

S

T

U

V

W

X

Y

Z

   
EFFLUENT: the liquid (mobile phase) that leaves the column.


 
ELECTROCHEMICAL DETECTOR: a less common LC detector that can be very sensitive for some compounds, such as catecholamines. The detector works by oxidizing or reducing sample compounds.


ESA 5200 Electrochemical Detector


   
ELUANT: another name for MOBILE PHASE.


 
ELUTE: to wash a band from a column. Or the action of washing from the column, as "the band eluted with a retention time of 5.0 minutes".


 
END-FITTING: the metal part that closes off the end of the column and connects to the tubing through which mobile phase enters and leaves the column. The end-fitting contains a frit that keeps the packing inside the column.


Generic Column End Fitting Cutaway


   
EXTERNAL STANDARD METHOD or EXTERNAL STANDARDIZATION: a calibration procedure that does not add an internal standard to the samples to be assayed. The size of the analyte peak in the sample is compared directly to the size of the analyte peak in the calibrator.


 
   
 

 


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Last revised: April 02, 2001.