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| MAIN COLUMN:
See Analytical Column.
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| MEGAPASCAL (MPa): a unit
of pressure; one MPa equals about 10 bar (atmospheres) or
150 pounds per square inch (psi).
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| METHANOL: one of
three organic solvents commonly used (with water) to
prepare reversed-phase mobile phases (see also ACETONITRILE and TETRAHYDROFURAN).
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| MICROGRAM (µg): a unit
of mass, equal to one millionth of a gram. See Section 4B for the relationship to other units
of mass.
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| MICROLITER (µL): a unit
of volume, equal to 0.001 milliliter. See Section 4B for the relationship to other
volume units.
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| MICRON (µm): a
thousandth of a millimeter or millionth of a meter;
particles used as HPLC column packings have diameters of
3 to 10 microns (0.0001-0.0003 inches) for analytical
columns.
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| MILLIGRAM (mg): a unit
of mass, equal to 0.001 gram. See Section 4B for the relationship to other units
of mass
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| MILLILITER (mL): a unit
of volume, equal to 0.001 liters. See Section 4B for the relationship to other
volume units.
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| MISCIBLE: two
solvents are miscible if they combine with each other in
all proportions to form a single phase; water and
methanol are miscible, hexane and water are not miscible.
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| MOBILE PHASE: the
liquid that flows through the LC system and column; it
carries the sample from the column inlet to the detector.
The mobile phase is usually a mixture of two or more
solvents.
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MOBILE PHASE STRENGTH: determines how fast the sample
moves through the column; a STRONG MOBILE PHASE results
in sample bands coming out fast, and a WEAK MOBILE PHASE
gives longer retention times for each band. In the
reversed-phase example shown below, 40% methanol/water is
a stronger mobile phase than 30% methanol/water.
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| MODULE: a sub-unit
of an HPLC system. The pump, injector and detector are all modules.
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