Getting Started in HPLC

Section 0. The Language of HPLC: B

A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

S

T

U

V

W

X

Y

Z

   
BAND: The distribution of an analyte from the sample in the chromatographic system. When the band passes through the detector, the result is a PEAK. In practice, the two terms are often used interchangeably


This chromatogram has 6 bands (peaks)


   
BAND SIZE: See Peak Height or Peak Area.


 
BAND WIDTH: The width of the band measured at its base. Also called BASELINE WIDTH.


The "Baseline" width of a peak misses some area at the front and back!


   
BAR: in HPLC a unit of pressure, equal to one atmosphere; also equal to about 15 pounds per square inch (psi) or 0.1 Megapascal.


 
BASELINE: the response of the detector on the data system display when no sample band is passing through the detector.


The "baseline" is the detector response when no sample is eluting from the column.


   
BASELINE DRIFT: a baseline that is not perfectly horizontal, but rises or falls with time.


In the "real world", not all baselines are flat and horizontal.


   
BASELINE WIDTH: The width of the band measured at its base. Also called BAND WIDTH.


 
BATCH NUMBER: See LOT NUMBER.


 
BINARY-SOLVENT MOBILE PHASE: a mobile phase composed of two solvents, such as methanol plus water. Most mobile phases are of this type. Also called BINARY MOBILE PHASES.


 
BODY: refers to the outside of a fitting, into which a nut screws. Also called a Union. Most fittings used in HPLC are characterized as "ZDV" (Zero Dead Volume).


Upchurch tube end & fitting body (ZDV union)


   
BONDED PHASE: the layer of organic material that covers the surface of the pores inside of the stationary phase particle, and is responsible for the separation characteristics of an LC column.


 
   
 

 


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Last revised: April 02, 2001.