Getting Started in HPLC

Section 0. The Language of HPLC: G

A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

S

T

U

V

W

X

Y

Z

   
GEL FILTRATION CHROMATOGRAPHY (GFC): A form of Size-Exclusion Chromatography (SEC) using aqueous mobile phases and hydrophilic stationary phases. Commonly used for separations of proteins or nucleic acids on the basis of molecular size.


 
GEL PERMEATION CHROMATOGRAPHY (GPC): A form of Size-Exclusion Chromatography (SEC) using organic mobile phases and hydrophobic stationary phases. Commonly used for separations of synthetic polymers on the basis of molecular size.


 
GRADIENT ELUTION: an HPLC procedure where the composition of the mobile phase changes during the separation. For example, the mobile phase starts out as water, and methanol is added continuously so that the percent methanol in the mobile phase increases from zero to 100% during the run. See also ISOCRATIC.


 
GRAM (g): a unit of mass, equal to about 0.03 ounces. See Section 4B for the relationship to other units of mass.


 
GUARD COLUMN: a short column placed between the sample injector and the inlet of the main ("analytical") column; the guard column is packed with the same kind of packing as the main analytical column, and is intended to absorb or pick up impurities in the sample or mobile phase that might damage the main column.


Protecting the Analytical Column


   
 

 


2000, LC Resources Inc. All rights reserved.
Last revised: April 02, 2001.