Getting Started in HPLC

Section 0. The Language of HPLC: F

A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

S

T

U

V

W

X

Y

Z

   
FILLED-LOOP INJECTION: sample injection technique in which the loop of the injector is completely flushed out with sample (using 2 or 3 loop-volumes) before rotating the valve into the inject position. See also PARTIAL LOOP INJECTION.


In filled-loop injection, an excess of sample is flushed through the loop during the load process.


   
FITTINGS: refers to the connectors that join tubing, columns and various LC modules together.


This type of fitting is referred to as "zero-dead-volume" (ZDV), because the passage through the fitting is the same diameter as the inside of the tube.


   
FIXED-WAVELENGTH DETECTOR: a obsolete type of photometric detector having a limited selection of wavelength settings, generally selected by changing optical filters.


 
FLUORESCENCE DETECTOR: a less common detector that can be quite sensitive and specific for some compounds.


Waters 474 Fluorescence Detector


   
FLOW CELL: the part of a photometric detector through which the sample and mobile phase pass. The sample is detected in the flow cell.


UV Detector Flow Cell Cutaway


   
FRIT: a porous metal filter that is used to (a) prevent particulates from entering the LC system with the mobile phase, (b) to contain the column packing inside the column, or (c) to protect other parts of the LC system. Frits vary in their porosity from 0.5 micron (in-line filters for protecting the column) to 10 microns (inlet filters or "sinkers" attached to the pump inlet line).


 
   
 

 


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Last revised: April 02, 2001.