Getting Started in HPLC
Section 0. The Language of HPLC: T
| TAILING FACTOR: a
measure of how much a band deviates from being perfectly
bell-shaped or symmetrical; calculated as shown here. See
also ASYMMETRY FACTOR. In most cases, the ASYMMETRY
FACTOR and TAILING FACTOR for a peak will be of similar
magnitude, but only rarely are they exactly equal.
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The Tailing Factor is one way of describing tailing
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| TERNARY-SOLVENT
MOBILE PHASE: a mobile
phase composed of
three solvents (e.g., methanol, water and acetonitrile.)
Also called TERNARY MOBILE PHASE.
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| TEST
CHROMATOGRAM: a chromatogram from the column
manufacturer that usually accompanies a new column,
showing the separation of a particular sample under some
set of experimental conditions; the test chromatogram
shows how well the column performs under standard (and
generally ideal) conditions.
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| TETRAHYDROFURAN
(THF):
one of three organic solvents commonly used (with water) to
prepare reversed-phase mobile phases (see also ACETONITRILE and METHANOL).
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| TMS or
TRIMETHYLSILYL: a kind of bonded phase used for reversed-phase chromatography.
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| TROUBLESHOOTING:
refers to locating the cause of problems with the LC
equipment or procedure, and solving these problems.
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