Getting Started in HPLC

Section 0. The Language of HPLC: T

A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

S

T

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X

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Z

   
TAILING FACTOR: a measure of how much a band deviates from being perfectly bell-shaped or symmetrical; calculated as shown here. See also ASYMMETRY FACTOR. In most cases, the ASYMMETRY FACTOR and TAILING FACTOR for a peak will be of similar magnitude, but only rarely are they exactly equal.


The Tailing Factor is one way of describing tailing


   
TERNARY-SOLVENT MOBILE PHASE: a mobile phase composed of three solvents (e.g., methanol, water and acetonitrile.) Also called TERNARY MOBILE PHASE.


 
TEST CHROMATOGRAM: a chromatogram from the column manufacturer that usually accompanies a new column, showing the separation of a particular sample under some set of experimental conditions; the test chromatogram shows how well the column performs under standard (and generally ideal) conditions.


 
TETRAHYDROFURAN (THF): one of three organic solvents commonly used (with water) to prepare reversed-phase mobile phases (see also ACETONITRILE and METHANOL).


 
TMS or TRIMETHYLSILYL: a kind of bonded phase used for reversed-phase chromatography.


 
TROUBLESHOOTING: refers to locating the cause of problems with the LC equipment or procedure, and solving these problems.


 
   
 

 


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Last revised: April 02, 2001.