Getting Started in HPLC

Section 0. The Language of HPLC: H

A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

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T

U

V

W

X

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Z

   
HELIUM SPARGING: the bubbling of helium through the mobile phase to remove dissolved air. See DEGASSING.


 
HIGH-PRESSURE MIXING: a pumping procedure in which two or more different solvents are mixed on the high pressure side of the pump to form a final mobile phase. Generally one pump is required for each solvent, so such systems are often referred to as "two-pump" systems. See also LOW-PRESSURE MIXING.


Waters 2-pump gradient system


   
HPLC or HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY: a special application of liquid chromatography using instrumentation to allow complex separations and analyses to be carried out in very short times. Also often called HIGH-PRESSURE LIQUID CHROMATOGRAPHY, it is distinguished from "classical" or "open-column" liquid chromatography in which mobile phase flows through the column by gravity only. It is also called "LC" for short.


 
HYDROPHILIC: "water-loving"; refers to compounds, solvents or bonded phases that either dissolve easily in water or prefer water to nonpolar organic solvents. See HYDROPHOBIC.


 
HYDROPHOBIC: "water-hating"; refers to compounds, solvents or bonded phases that either dissolve easily in nonpolar organic solvents such as hexane, or prefer such solvents to water. See HYDROPHILIC.


 
   
 

 


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Last revised: April 02, 2001.