Getting Started in HPLC

Section 0. The Language of HPLC : V

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VACUUM DEGASSING: removal of air from the mobile phase or a solvent by applying vacuum to the liquid so that air bubbles out. See also DEGASSING.


  
VARIABLE-WAVELENGTH DETECTOR: a photometric detector that allows the selection of any wavelength setting for detection (usually 190 to 360 nm).


  
VISCOSITY: the property of a solvent or mobile phase that tells how hard it is to pump that solvent through a pipe or column; solvents such as hexane or acetonitrile have low values of viscosity (measured in centiPoise, cP) and pump easily with low pressures. Liquids such as molasses are hard to pump and have very high values of viscosity.


 
VOID or COLUMN VOID: a hole in the column packing, generally at the inlet of the column, or also a settling of the packing at the column inlet to create a space between the top of the packing and the frit. A void usually makes the column unsuitable for use.


  
   
 

 

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Last revised: April 02, 2001.